Sample information

Caught on a bright and clear day in a pollen garden right outside the building where we have class. Caught by picking up with gloved hands and then putting into a bug net. Was caught around 1:00 PM, and was one of eight caught.

Methods

DNA Extraction: I removed a majority of the insect that wasn’t its abdomen so that I could primarily focus on the innards found exclusively in the abdomen. I then took great care to crush it up thoroughly in a solution until I saw minimal/no free-floating big clumps of debris.

Lanes:

1. 100bp Ladder
2. Empty
3. American Bird Grasshopper Sample 2 Arthropod Primers
4. American Bird Grasshopper Sample 2 Wolbachia Primers
5. Goldenrod Soldier Beetle Sample 3 Arthropod Primers
6. Goldenrod Soldier Beetle Sample 3 Wolbachia Primers
7. American Bird Grasshopper Sample 3 Arthropod Primers
8. American Bird Grasshopper Sample 3 Wolbachia Primers
9. Empty
10. Arthropod Positive Control
11. Arthropod Negative Control
12. Wolbachia Positive Control
13. Wolbachia Negative Control

Analysis: My controls all worked, and while it’s faint, you can see bands for both the Arthropod DNA and the Wolbachia DNA, showing that Wolbachia can be present in Schistocerca americana.

Results

I put medium as both the bands for the arthropod and Wolbachia DNA are kinda faint. Controls all worked though.