Ant

May 4, 2021

Sample information
Picture
Photos by: Tucker G
Location
Collection date 04/02/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

I noticed that the Ant was sort of clear looking under the microscope, had black eyes and looked like it had a flat bottom half. Another thing is that it had six legs, had a circle band in side of the stomach.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My pellet was brown, it was actually pretty big and noticeable, I disturbed it once so I had to restart that process but then I finally got it.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

It was low because my hand kept shaking while putting the liquid inside of the gel. I also Disturbed the pellet once.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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