Odonata

May 4, 2021

Sample information
Picture
Location
Collection date 04/04/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Small black flying arthropod with 2 wings and 4 legs. I found it in my car on the way to school and it was about 60°
Putative identification Arthropoda Hexapoda Insecta Odonata

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

My pellet was small and white and was barely visible. I messed up the gels while making them and poked through creating problems with the Wolbachia test.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

not sure about the wolbachia DNA band and arthropod negative control
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Odonata was found to be postive for Wolbachia.
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