Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/04/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | Small black flying arthropod with 2 wings and 4 legs. I found it in my car on the way to school and it was about 60° |
| Putative identification | Arthropoda Insecta Odonata |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | My pellet was small and white and was barely visible. I messed up the gels while making them and poked through creating problems with the Wolbachia test. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | not sure about the wolbachia DNA band and arthropod negative control |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Odonata was found to be postive for Wolbachia. |
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid
Blattella germanica