Fruit Fly

May 5, 2021

Sample information
Picture
Location
Collection date 03/03/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

The Arthropod has 3 sections. It has six legs. It also has a pair of wings; the wings are split into two sections. It also has hair all around its body including the head. It has a long nose. It also has red eyes which occupy the majority of the head.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The pellet is translucent around perimeter
It has a dark brown center
It also has a white layer between the center and the outside

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

DNA ladder in arthropod gel was not properly loaded; positive and negative controls did not work. Wolbachia gel control did not work; maybe problems with DNA extraction.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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