|Captive / Cultivated?||Wild-caught|
|Group||Hampton Roads Academy|
The bug has a large abdominal area, a pair of antennae, 6 legs, and sturdy wings.
|Putative identification||Hexapoda Insecta Lepidoptera|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Abdomen|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniOne|
My pellet was barely visible after centrifuging the tubes for 5 minutes. In the moment I thought I had messed up somehow, but it turned out that the pellet was just a bright color, therefore making it difficult to see. I also feel as though I forgot to set the pipette to the right measurements when using it.
|Explanation of confidence level||
DNA ladder in arthropod gel was not properly loaded. Positive and negative controls did not work. Wolbachia gel positive control did not work; there may have been problems with the DNA extraction.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Lepidoptera was found to be negative for Wolbachia.|