Ant

May 5, 2021

Sample information
Picture
Location
Collection date 06/27/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

antennas, 6 legs, and black
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Not enough supernatant and pipetted tiny tubes wrong. The pellet was small but visible.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

DNA ladder in arthropod gel was not properly loaded. Positive and negative controls did not work. Wolbachia gel control did not work, may be problems with the DNA extraction.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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