Wolbachia Lab: DNA Extraction

Sample information

Picture
Location
Collection date 10/06/2025
Captive / Cultivated? Captive / Cultivated
Group Wolcott College Prep
Observations

Fly- Black with wings

Nat- Small bug with wings and white

Putative identification

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

I feel confident because the outcome was good and nothing was done wrong in the procedure.

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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