Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/05/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | This bug was found in some dirt and rocks. Specifically, it was located under a brick. Many types of bugs were found under there but I picked the biggest. On this day it was overcast and windy. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | Followed wolbachia protocol |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | We are pretty confident, but no 100% sure |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Armadillidium vulgare
Bee
Ground Beetle
House Cricket 8A
6J Ant