Millipede 7F

February 17, 2026

Sample information
Picture
Entry by: Luca K
Location
Collection date 09/10/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Captured in wood chips, a slender, black, circular body appears to have plating or a protective shell. The bug also has many legs.
Putative identification Arthropoda Diplopoda Julida Julidae Cylindroiulus Cylindroiulus caeruleocinctus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

Followed Wolbachia Project Protocols

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Cylindroiulus caeruleocinctus was found to be negative for Wolbachia.
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