Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/05/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | Absolutely tiny black ants living in a colony of thousands in dirt and woodchips next a man made pond near a house. Similar ants have been seen making colonies of similar size in sidewalk just around the block. Tiny black ants with large abdomen connected with small nodes. Thorax is larger compared to the abdomen than one of a little black ant, giving me confidence that this is a pavement ant |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Tetramorium Tetramorium immigrans |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | |
| Gel images |
|
| Protocol notes | I was in class and we did this project and the teacher gave us a “PCR cocktail” that has all of the ingredients for PCR to work but was also colored green. The Gel electrophoresis was done with other students in the same gel and my DNA was in the circles area. I have the arthropod bar but no wolbachia bar |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am very confident because we did all the steps according to plan, all the controls worked well, and I never had any issues while extracting DNA |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Tetramorium immigrans was found to be negative for Wolbachia. |


Soil Centipede
Brown Millipede
Greenhouse Millipede
Black Ant