Ground Beetle

January 27, 2026

Sample information
Picture
Entry by: Leo M
Location
Collection date 09/05/2025
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Black six legged beetle
Putative identification Arthropoda Insecta Coleoptera Carabidae Poecilus Poecilus chalcites

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer Other
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Controls worked correctly and there is no reason to think there is a problem with the results
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Poecilus chalcites was found to be negative for Wolbachia.
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