Mosquito 1

January 14, 2026

Sample information
Picture
Entry by: NNC
Location
Collection date 09/29/2025
Captive / Cultivated? Wild-caught
Group Rutgers Preparatory School
Observations

Cloudy, 62 degrees F, 1:50pm, slight wind. Found on the ground by gazebo. Caught on dirt and grass.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer 1X TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

Standard protocol

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Out of the 2 times we did the protocol, we successfully gotten a negative Wolbachia infection. Everything else went smoothly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium was found to be negative for Wolbachia.
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