Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/05/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Hamburg Area High School |
| Observations | Slender, dirt-colored insect about less than half an inch long with a dark, elongated head and a distinct/long neck. |
| Putative identification | Arthropoda Insecta Hemiptera Rhyparochromidae Myodocha Myodocha serripes |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | agarose gel electrophoresis |
| Buffer | TBG |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Standard |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | There is a possibility of contamination or misread results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Myodocha serripes was found to be postive for Wolbachia. |


Wolbachia Project
Alexander Devlin-Myrmica
Brookelynn Foote- Coccinellidae
Maile Bentz- Harmonia axyridis
Madison Adams – Harmonia axyridis