Olivia Kauffman Coccinellidae

January 29, 2026

Sample information
Picture
Entry by: Olivia K.
Location
Collection date 11/09/2025
Captive / Cultivated? Wild-caught
Group Hamburg Area High School
Observations

small, round beetle with a bright, colorful shell. Smooth, shiny, dome-shaped body with red or orange with black spots on outer wing.
Putative identification Arthropoda

Methods
Extraction kit QIAGEN DNeasy kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

My results were verified by my teacher.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
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