January 30, 2026

Sample information
Picture
Entry by: Mackenzie H.
Location
Collection date 11/09/2025
Captive / Cultivated? Wild-caught
Group Hamburg Area High School
Observations

brown body, two segments, 2 cm in length, 8 legs
Putative identification Arthropoda Arachnida Araneae Lycosidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer TBG
DNA stain SYBR Safe
Gel images
Protocol notes

Standard

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

There may have been too much excess tissue/debris in the tube when extracting the DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lycosidae was found to be negative for Wolbachia.
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