Millipede

February 9, 2026

Sample information
Picture
Entry by: Sophia Severns
Location
Collection date 10/02/2025
Captive / Cultivated? Wild-caught
Group Penn State University
Observations

I found the millipede under some moss near a tree stump at the Penn State golf courses. It was in a moist environment under the moss. The weather was a nice sunny day, however, the millipede was under shade in darkness.
Putative identification Arthropoda Diplopoda Polydesmida Polydesmidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

DNA extraction: The entire arthropod was crushed in the tube, including the exoskeleton because it was very small.

Gel Electrophoresis Lanes

Lane Substance
1 DNA Ladder
2 Partner arthropod
3 My arthropod (millipede)
4 Positive Control
5 Negative control
6 (+) DNA
7 (-) water

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The gel electrophoresis showed an arthropod band for my arthropod, but no Wolbachia band.  The only band that was seen in my arthropod lane was the top line which is the arthropod line. My arthropod matches with the fifth lane which is the negative control, so I can conclude that my arthropod does not have Wolbachia. Both the controls worked well and I was able to compare my millipede arthropod to the control data.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Polydesmidae was found to be negative for Wolbachia.
Report Inappropriate Post