Harmonia axyridis, New Marlborough MA

May 14, 2026

Sample information
Picture
Entry by: Malcolm A
Location
Collection date 02/23/2026
Captive / Cultivated? Wild-caught
Group Berkshire Community College
Observations

Strange behavior amongst others, they clump together on the walls and windowsills.
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We used a DNA extraction kit based on the insect adaptation of New England Biolab’s Monarch Spin gDNA Extraction kit. (Product t3010)

The specimen was incubated in a water bath at 56 degrees C for 60 minutes.

Our first gel image was taken on 3/24/2026 and was run at 125 volts for 20 minutes. We used the New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # no559s)

Our second gel image was taken on 4/7/2016 and was run at 125 volts for 37 minutes. We used the New England Biolabs 1 kb Plus DNA Ladder for Safe Stains (product # no559s)

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I am reasonably confident that my sample is positive. The positive and negative controls line up and are clearly visible which makes me confident that my results are accurate. A single PCR reaction should be run for higher confidence. I am confident for both rounds of PCR.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Harmonia axyridis was found to be postive for Wolbachia.
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