Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/12/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | In a forest under a grey stone on the ground near sprouces |
| Putative identification | Arthropoda Malacostraca Isopoda Trichoniscidae Trichoniscus Trichoniscus pusillus |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | agarose gel electrophoresis |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The control did work at both my lanes. It showed the barriers |
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence |
|
| Summary | The Trichoniscus pusillus was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR