Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 07/06/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Norwich Free Academy |
| Observations | Has a black face, white butt, grey scale like skin, six legs, and an orange bump above its head. |
| Putative identification | Arthropoda Insecta Coleoptera Scarabaeidae Phyllophaga |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | The bug is in the fourth well. I extracted the DNA by crushing the whole bug in the tube. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | My control didn’t show up so I don’t know if there is any or not. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Phyllophaga was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid