Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/26/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Gymnasium Immensee |
| Observations | No special observations. |
| Putative identification | Arthropoda Insecta Diptera Lipoptena Lipoptena cervi |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | 1X TAE |
| DNA stain | Cyber Green |
| Gel images |
|
| Protocol notes | column 1) Is the examined fly, shows only one strip at the insect DNA level. 3) (Not visible) is the positive test. 4) Is the negative test 5) Wolbachia example 6) water 7) DNA-ladder |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | The postive test didn`t work properly. And the DNA-ladder are extremely strong. The negative conrol showed a positive result. negative control- insect without wolbachia positive control- insect with Wolbachia |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Lipoptena cervi was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR