BRO-IJS Diptera

April 14, 2026

Sample information
Picture
Entry by: Isaiah S
Location
Collection date 01/27/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected out of a bush using a beat net, forceps and a jar.
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

No major experimental flaws were present and the results were straight forward.
Wolbachia 16S sequence
GTGCTGATCCACGATTACTAGCGATTCCAACTtccttttctCGAGTTGCA GAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGCG ACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCC ATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCA CTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATG AGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTG ACGACAGCCATGCAACACCTGTGTGAAACCCGGCCGAACCGACCCTATCC CTTCGAATAGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GTAGGAACATCTCTTAGAATCTTAATCCGAGCAGAATTAGGACATCcatg aGCATTAATTGGAGATGACCAAATTTATAATGTAATTGTAACTGCTCATG CATTTATTATAATTTTCTTTATAGTAATACCTATTATAATTGGTGGATTT GGTAACTGATTAGTTCCTTTAATACTAGGAGCTCCAGATATAGCCTTCCC CCGAATAAATAATATAAGTTTTTGGTTACTACCACCAGCTTTAACTTTAC TCCTAGCAAGCAGTATAGTAGAAAACGGAGCTGGTACAGGTTGAACTGTT TACCCTCCCCTATCTTCTAATATCGCCCATGGAGGAGCTTCTGTAGATTT AGCTATTTTCTCTTTACACTTAGCTGGAATTTCTTCAATTTTAGGAGCAG TAAATTTTATTACTACTGTAATTAATATACGATCTACAGGAATTACCTTT GATCGAATACCTTTATTTGTTTGATCCGTAACTATCACAGCTTTATTATT ACTTTTATCTTTACCAGTATTAGCAGGAGCTATCACAATACTATTAACAG ATCGAAATTTAAATACATCTTTCTTTGACCCCGCTGGAGGAGGAGATCCA ATTTTATATCAACACCTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be postive for Wolbachia.
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