Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/22/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Seems to be ant with wings, mandible, and 3 parts to the body |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The insect matched what was predicted that it would be Order Hymenoptera being that the DNA extraction steps were followed closely to ensure the results were correct. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TTTGAGCAGGAATAATTGGTTCATCTATAAGAATAATCATCCGATTAGAA CTAGGATCTTgttattCTCTAATTAATAATGATCAAATCTACAACTCCCT AGTTACTAGACACGCTTTTATTATAATTTTTTTTATAGTTATGCCCTTCA TAATTGGGGGATTTGGAAATTTCTTAGTCCCCTTAATACTCGGCTCCCCT GATATGGCCTATCCTCGAATAAATAATATAAGATTTTGACTTCTACCACC TTCTCTAACTCTTTTACTTATCAGAAGATTTATTAATAGAGGAGTGGGGA CAGGATGAACTATCTATCCACCACTAGCCTCCAATATTTTTCACAGAGGG GCCTCTATTGACCTATCTATTTTTTCCTTACATATCGCCGGAATATCATC AATCTTAGGGGCTATTAATTTTATTTCTACAATCATTAACATACACCATA AAAATTTTACTATAGATAAAATCCCCCTACTAGTCTGATCTATCCTTATT ACAGCCATTCTTCTTTTACTTTCCCTCCCAGTTCTTGCAGGAGCTATTAC AATATTACTAACTGACCGTAATTTAAACACCTCCTTCTTCGATCCCTCGG GAGGGGGAGATCCCATTCTATATCAACATTTATTCTGATTTTTTGGTCAC C
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
DEM-AES – Hymenoptera
DEM-ORK Order Hymenoptera
(no title)
DEM-OKA Orthoptera
DEM-MGC Coleoptera