Ear Wig

July 16, 2021

Sample information
Picture
Photos by: Aubrey R.
Location
Collection date 07/07/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

It has a brown head, black body, and brown the rest of the way down. It has pinchers at the rear end and six legs. Two antennas and has a shiny casing. It was found in a leaf pile by a river.
Putative identification Arthropoda Insecta Dermaptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

The ear wig is in well six. I used the abdomen because the rest of the body wasn’t necessary.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

My control didn’t show up on the gel.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Dermaptera was found to be negative for Wolbachia.
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