|Captive / Cultivated?||Wild-caught|
|Group||Norwich Free Academy|
The grub is tan with a orange head and black mouth. The grub has six legs that are all located in the beginning of the body. The bug has a shiny coat over him.
|Putative identification||Hexapoda Insecta Coleoptera Scarabaeidae Phyllophaga|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Standard electrophoresis system|
|DNA stain||Fast Blast|
I used the entire arthropod. It is located in the fifth well.
|Explanation of confidence level||
This bug was positive with Wolbachia, but my confidence is low because the positive control didn’t show up.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Phyllophaga was found to be postive for Wolbachia.|