Grub

July 16, 2021

Sample information
Picture
Photos by: Nora G
Location
Collection date 07/06/2021
Captive / Cultivated? Wild-caught
Group Norwich Free Academy
Observations

The grub is tan with a orange head and black mouth.  The grub has six legs that are all located in the beginning of the body.  The bug has a shiny coat over him.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Scarabaeidae Phyllophaga

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

I used the entire arthropod. It is located in the fifth well.

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

This bug was positive with Wolbachia, but my confidence is low because the positive control didn’t show up.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Phyllophaga was found to be postive for Wolbachia.
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