Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 07/06/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Norwich Free Academy |
| Observations | The grub is tan with a orange head and black mouth. The grub has six legs that are all located in the beginning of the body. The bug has a shiny coat over him. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Scarabaeidae Phyllophaga |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | I used the entire arthropod. It is located in the fifth well. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | This bug was positive with Wolbachia, but my confidence is low because the positive control didn’t show up. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Phyllophaga was found to be postive for Wolbachia. |
Water Boatman