Enallagma civile

April 30, 2026

Sample information
Picture
Entry by: Aliza Z
Location
Collection date 09/04/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Fall 2026
Putative identification Arthropoda Insecta Odonata Coenagrionidae Enallagma Enallagma civile

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

DNA sequence
Wolbachia 16S sequence Download FASTA    Download AB1
ACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCnTTAAAAGCCA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download AB1
TGGATCATTAATTGGAGATGACCAGATTTATAATGTAGTAGTTACTGCACATGCATTTGTTATAATTTTCTTTATAGTTA TACCCATTATAATTGGAGGATTTGGAAATTGACTGGTACCATTAATATTAGGAGCACCAGATATAGCTTTCCCCCGATTA AATAACATGAGATTCTGACTTTTACCCCCATCACTAACCTTATTACTAGCAAGAAGATTAGTAGAAAGAGGGGCTGGGAC CGGGTGAACCGTATACCCACCATTAGCAGGTGTAATCGCTCATGCTGGGGCATCCGTTGATTTAACTATTTTTTCACTAC ACTTAGCAGGAGTATCATCAATTTTAGGAGCAATTAATTTTATTACAACTACTATTAATATAAAGTCACCAGGTATAAGT ATAGATCAACTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Enallagma civile was found to be postive for Wolbachia.
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