Bee – Basd

May 4, 2026

Sample information
Picture
Entry by: Caleb K.
Location
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Beaver Area School District
Observations

The identity of this insect is not certain, however, it was discovered on the grill of my car. I began my swabbing it off of my vehicle and placing it in a tube. It was collected at around 8:00 and the temperature was around 59 degrees F.
Putative identification Arthropoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer TAE
DNA stain UView
Gel images
Protocol notes

I dissected the abdomen—which was hollow— and made sure to crush it up. I didn’t include the exoskeleton. Lane one is a DNA ladder, two is my bee, three is my house fly, four is a positive fruit fly, five is a negative fruit fly, six is a positive DNA, and seven is water.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

There could be a possible lane spillover, because there also appears to be DNA for the negative fruit fly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be postive for Wolbachia.
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