Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/26/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Beaver Area School District |
| Observations | My insect was caught on a car in Pennsylvania, in the school parking lot. It was fair weather about 65 degrees in the morning. |
| Putative identification | Arthropoda Insecta Diptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | BioRad |
| Buffer | TAE |
| DNA stain | UView |
| Gel images |
|
| Protocol notes | The insect was so small that I couldn’t directly get the DNA out, so I placed the insect in and smashed it up. Lane 2:insect 1(flying ant) Lane 3:insect 2(this sample:gnat) Lane 4:positive fruit fly Lane 5:negative fruit fly Lane 6:positive DNA Lane 7:water |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am highly confident due to the fact that all my controls came out normal, with no unusual result. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR