Enallagma annexum (Pond Damselfly)​

May 6, 2026

Sample information
Picture
Entry by: Alisha S.
Location
Collection date 09/03/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Collected by Fall 2025 students
Putative identification Arthropoda Insecta Odonata Coenagrionidae Enallagma Enallagma signatum

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Last two segments and second segment
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There was minor RNA and salt contamination within the sample. Not 100% sure of results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Enallagma signatum was found to be negative for Wolbachia.
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