Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 04/10/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | This spider was found inside a residence. |
| Putative identification | Arthropoda |
Methods |
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| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
| Buffer | 1X TAE |
| DNA stain | UView |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level |
Nothing appeared in the positive and negative wolbachia wells; therefore wolbachia cannot be confirmed. However, the DNA only had one band that matched the DNA+ control, leading us to believe that the DNA indicated arthropod status and not Wolbachia presence. The DNA Positive Control was visible, but the Wolbachia Positive DNA was not visible. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
AGCTTGNTCTGCTATAGCTGGAACAGCTATAAGAATTTTAATTCGAATGGAATTAGGACAGTTAG GATCTTTTTTAGGAGATGATCAATTATATAATGTTGTAGTAACTGCTCATGCTTTTGTTATAATT TTTTTTATAGTAATACCAATCTTAATTGGTGGTTTTGGAAATTGATTAGTTCCATTAATATTAGG AGCACCTGATATAGCTTTCCCTCGAATAAATAATTTAAGATTTTGACTTTTACCTCCTTCTTTAT TTTTATTATTTATATCTTCTATAGTGGAAATAGGAGTTGGTGCTGGATGAACTGTGTATCCTCCT TTAGCATCAGTTATAGGACATGCTGGAAGTTCAGTTGATTTTGCTATTTTTTCTTTACATTTAGC TGGAGTATCTTCAATTATAGGAGCTATTAATTTTATTTCTACAATTATTAACATACGCTTATTTA GAATATCTATGGAAAAGATTCCTTTATTTGTATGATCAGTATTAATTACTGCTATTTTATTATTA TTGTCTTTACCTGTATTAGCAGGTGCTATTACTATATTATTAACTGATCGAAACTTTAATACTTC TTTTTTTGATCCTGCTGGAGGGGGTGATCCTATTTTATTTCAACATTTATTTTGATTTTTTGGTC ACCCTNGAAAGTTTAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Arthropoda was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR