Juvenile Cobweb Spider GROUP2

May 22, 2026

Sample information
Picture
Entry by: Mekayla J
Location
Collection date 04/23/2026
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations
  • Oval shaped body
  • Eight short legs
  • Two eyes
  • Light brown
  • Harmless Arachnids
Putative identification Arthropoda Arachnida

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain UView
Gel images
Protocol notes

Edwards Buffer was used in the DNA extraction

Duplex PCR performed with the cocktail and F/R primers

Gel electrophoresis with 1x TAE buffer and ran at 120V, 65 mA

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Able to get all of my controls (minus the + DNA control since I already had it), and DNA Ladder was present
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arachnida was found to be postive for Wolbachia.
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