Sample information

Sighted flying around a persimmon tree and eventually flew into the center of it, landing under a leaf. Once caught, it rapidly started flying around within the container.

Methods

DNA Extraction Protocol

Gel Electrophoresis Protocol

Results

After we ran gel electrophoresis and loaded all of the samples we put the gel on a UV light box in order to see the bands. Our ladder produced 5 bands, indicating a successful run as well as our positive control which produced 2 bands. The arthropod gene has an expected band size of 708 bps while the wolbachia gene has 438 bps. As seen on the gel the band closest to the wells is the band with more base pairs, in this case would be indicating the sample is an arthorpod. Because the lane we loaded our moth sample only produced one band that aligned with the band from the positive control, we can be confident that our moth was an arthropod and not wolbachia infected.