Winterset Arthropod 4

May 26, 2026

Sample information
Picture
Entry by: Addison G
Location
Collection date 04/21/2026
Captive / Cultivated? Wild-caught
Group Winterset High School
Observations

Multicolor, 8 legs, small, found under a rock, wooded area
Putative identification Arthropoda Arachnida Araneae Gnaphosidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TBE
DNA stain Cyber Green
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I felt confident about this extraction because I followed the protocol.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Gnaphosidae was found to be postive for Wolbachia.
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