Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/10/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | Medium sized bee |
| Putative identification | Arthropoda Insecta Hymenoptera Apidae Apis Apis mellifera |
Methods |
|
| Extraction kit | Qiagen Blood and Tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | DNA extraction likely didn’t work because the bands didn’t appear in the gel electrophoresis results for this lane with this specific sample |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | We didn’t see a band that showed up with our positive Wolbachia control, but we also didn’t see the first DNA band, which demonstrates that the DNA extraction was unsuccessful in the sample in that specific lane. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR