Wolbachia Project

September 10, 2026

Sample information
Picture
Entry by: Mohammad S
Location
Approximate location: 36.14, -86.80
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

I observed a long body (tail) mosquito looking arthropod named Shellac Back Pixie. It has a brown long body with minimal dots.
Putative identification Arthropoda Insecta Diptera Asilidae Tipulogaster Tipulogaster glabrata

Methods
Extraction kit Blood and Tissue kit
DNA extraction location body without wings
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain SYBR Green
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The confidence level would be considered high due to our PCR results.
Wolbachia 16S sequence
Arthropod COI sequence
1-DNA ladder 2-H-1 Arthropod 3-H-2 Arthropod 4-Positive arthropod control 5-Negative arthropod control 6-Positive DNA control 7-Water
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Tipulogaster glabrata was found to be negative for Wolbachia.
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