Leiobunum sp. (Harvestmen Spider)

September 10, 2026

Sample information
Picture
Entry by: Anna M
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Spider with long legs and a small, orange body.
Putative identification Arthropoda Arachnida Opiliones Sclerosomatidae Leiobunum

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Our data was at the top of our gel. The first column was the DNA Standard/ladder, then arthropod 1, then arthropod 2, then positive control, then negative control, then DNA control, then water, then empty.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no banding for our Gel Electrophoresis Lab, therefore it is highly unlikely our arthropod has wolbachia. We diligently followed the protocols for the labs prior to the Gel Electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GGGGTTTGAGCTGCCATAGTAGGGTCAGCCCTAAGAATTCTTATTCGAACAGAGTTAGGCCAACCAGGGTCACTAATAAA TGATGACCAAATCTACAACGTAGTTGTTACTTCCCATGCTTTTGTGATAATTTTCTTCATAGTTATGCCCATTATAATAG GAAGTTTTGGAAACTGGCTAGTCCCTTTAATACTAGGGGCACCCGATATAGCCTTCCCTCGATTAAACAATATAAGATTT TGGTTGTTACCTCCTTCATTCATTCTTCTAATCAGCTCTACAATAGTAGAAAGAGGGGCAGGAACAGGATGAACTGTCTA TCCCCCCCTTTCAAGAAATACAGCTCATAGAGGACCCTCAGTAGATCTAACTATTTTTTCTCTTCATTTAGCCGGAATCT CCTCAATTTTAGGTGCTATCAATTTTATCACTACTGTAATGAACATACGAACAAAGGGGATAGTCTATGAACGTCTACCC CTCTTTGTCTGATCTATCATTATTACTGCTGTTCTTCTATTGCTGTCACTTCCAGTCTTAGCAGGGGCTATTACTATACT CCTTACAGATCGTAATTTCAACACATCTTTCTTTGACCCAGCAGGAGGAGGTGACCCTATCTTATACCAACATTTATTCT GATTTTTNNNNNNNNNNNNNAANNNTNNNNAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Leiobunum was found to be negative for Wolbachia.
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