Leaf- footed bug

September 10, 2026

Sample information
Picture
Entry by: Aqsa b
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Brown bug with 6 legs, long antennae, wings, and bigger back legs. It was found on a plant and moved by walking
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard election
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes
Group 2D (Aqsa and Jeanne)
Lanes 1-8

Lane 1- DNA Standard

Lane 2- Arthropod #1 Wol + COI1

Lane 3- Arthropod #2 Wol + COI1

Lane 4- Wol Pos Wol + COI1

Lane 5- Wol Neg Wol + COI1

Lane 6- gDNA control Wol + COI1

Lane 7- water control Wol + COI1

Lane 8- Empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

When we completed the Gel than bands did not show which indicate my organism was not Wolbachia positive
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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