Delia Platura – Fly

September 10, 2026

Sample information
Picture
Entry by: Matthew Gonzalez
Location No map location data available.
Collection date
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Lane 1: DNA Standard

Lane 2: Arthropod #1 Wol + Col1

Lane 3: Arthropod #2 Wol +Col1

Lane 4: Wol pos Wol + Col1

Lane 5: Wol neg Wol + Col1

Lane 6: gDNA control + Col1

Lane 7: Water Control Wol + Col1

Lane 8: EMPTY

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GNNTGGTCAGGAATAGTGGGAACCTCATTAAGTATTTTAATTCGAGCTGAATTAGGGCACCCTGGAGCATTAATTGGAGA TGATCAAATTTATAATGTAATTGTAACAGCTCATGCTTTTATTATAATTTTCTTTATAGTAATACCTATTATAATTGGAG GATTTGGAAATTGACTAGTTCCTTTAATATTAGGTGCCCCAGATATAGCTTTCCCTCGAATAAATAATATAAGTTTTTGA CTTCTACCTCCTGCATTAACTTTATTGTTAGTAAGAAGTATAGTAGAAAATGGAGCTGGGACAGGATGAACTGTTTACCC ACCTTTATCTTCTAATATCGCTCATGGCGGAGCTTCTGTTGATTTAGCAATTTTTTCTTTACATTTAGCAGGAATTTCAT CAATTTTAGGAGCCGTAAATTTTATTACTACTGTAATTAATATACGATCTACAGGAATTACTTTTGATCGAATACCTTTA TTTGTTTGATCAGTAGTAATTACAGCATTACTTCTTTTACTATCTTTACCGGTATTAGCTGGTGCCATCACTATACTATT AACAGATCGAAATCTAAATACATCATTTTTTGACCCTGCAGGAGGAGGAGATCCAATTTTATACCAACATTTATTTTGAT TTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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