Halyomorpha halys (Shield Bug)

September 10, 2026

Sample information
Picture
Entry by: Analiese S.
Location
Approximate location: 36.14, -86.80
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

The bug was frozen for a couple months.  It is brown and very crunchy.  Pestling it was a little difficult.
Putative identification Arthropoda Insecta Hemiptera Pentatomidae

Methods
Extraction kit Qiagen DNeasy Blood + Tissue Kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system standard electro
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

My specimen was in the bottom row: these are the numbers of the lanes from left to right:

9. DNA Standard

10. Arthropod#1Wol+Col1

11. Arthropod#2Wol+Col1

12. Wol(pos)Wol+Col1

13. Wol(neg)Wol+Col1

14. gDNA ControlWol+Col1

15. Water ControlWol+Col1

16. Empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My DNA sample did not have the defining Wolbachia band so I hypothesize that it did not have Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TNTTNGNNGNGTGAGCNNGAATAATTGGAACTTCTTTAAGTTTATTAATTCGTACAGAATTAGGTAATCCTGGATCACTA ATTGGAGATGATCAAATTTATAACACTATTGTTACAGCTCATGCCTTTATTATAATTTTTTTTATAGTTATGCCAATTAT AATTGGAGGATTTGGTAATTGATTAATTCCTTTGATATTAGGAGCCCCTGATATAGCTTTCCCACGTATAAATAATATAA GATTTTGATTACTACCCCCATCATTAACTNTATTAATTTCTAGAAGTATTGTTGAAAACGGAGCAGGAACAGGATGAACA GTTTACCCCCCTCTTTCCTCTAATATTGCCCATAGAGGATCTTCTGTTGATTTAGCTATTTTTTCTCTTCACCTTGCAGG AATTTCCTCTATCCTAGGAGCAATTAATTTTATTACAACAATTATCAATATACGAATTAATAATATGTCATTTGATCAAA TACCTTTATTTGTGTGAGCAGTAGGAATTACTGCTTTATTATTATTATTATCATTACCAGTTTTAGCTGGTGCAATTACT ATATTATTAACTGATCGGAATTTAAATACCTCTTTTTTTGACCCTGCTGGGGGAGGAGACCCAATTCTTTATCAACATTT ATTTTGANTTTNNNNNNNCCNNNNNANNANNNNNTANNAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Pentatomidae was found to be negative for Wolbachia.
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