Clouded Yellow (Lepidoptera)

September 10, 2026

Sample information
Picture
Entry by: Dylan N
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Flew fairly quickly, and blended into the greenery behind it.
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit Qiagen DNeasy Blood + Tissue Kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We covered the top row of lanes. Lane 1 held a DNA standard. Lane 2 held arthropod #1 Wolbachia + Col1. Lane 3 held arthropod #2 Wolbachia + Col1. Lane 4 held the Wolbachia positive control. Lane 5 held the Wolbachia negative control. Lane 6 held the gDNA control. Lane 7 held water. Lane 8 stayed empty.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

A band was present in lane 3, meaning that Wolbachia was present.
Wolbachia 16S sequence Download FASTA    Download AB1
TGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCAT CAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCC TTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAA GCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACG GTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCACTCGAAGCT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download AB1
GNNGNGTGAGCAGGAATAATTGGAACTTCTTTAAGTTTATTAATTCGTACAGAATTAGGTAATCCTGGATCACTAATTGG AGATGATCAAATTTATAACACTATTGTTACAGCTCATGCCTTTATTATAATTTTTTTTATAGTTATGCCAATTATAATTG GAGGATTTGGTAATTGATTAATTCCTTTGATATTAGGAGCCCCTGATATAGCTTTCCCACGTATAAATAATATAAGATTT TGATTACTACCCCCATCATTAACTTTATTAATTTCTAGAAGTATTGTTGAAAACGGAGCAGGAACAGGATGAACAGTTTA CCCCCCTCTTTCCTCTAATATTGCCCATAGAGGATCTTCTGTTGATTTAGCTATTTTTTCTCTTCACCTTGCAGGAATTT CCTCTATCCTAGGAGCAATTAATTTTATTACAACAATTATCAATATACGAATTAATAATATGTCATTTGATCAAATACCT TTATTTGTGTGAGCAGTAGGAATTACTGCTTTATTATTATTATTATCATTACCAGTTTTAGCTGGTGCAATTACTATATT ATTAACTGATCGGAATTTAAATACCTCTTTTTTTGACCCTGCTGGGGGAGGAGACCCAATTCTTTATCAACATTTATTTT GATTTTNNNNNNNNNNNNNANNNNTNNNAAAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be postive for Wolbachia.
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