JWO 07 – Clouded Sulphur Butterfly

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

Large flat wings, long antennae, yellow/green coloring.

On flower in HACC Pollinator Garden.
Putative identification Arthropoda Insecta Lepidoptera Pieridae Colias Colias philodice

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 7 on gel image.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Primer Dimers seen in lane but no Wolbachia or CO1 bands.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Colias philodice was found to be negative for Wolbachia.
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