JWO 11 – Fall Field Cricket

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.89
Collection date 09/03/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

On long strand pf grass in HACC Parking Lot

Dark brown body, long antennae, 2 hairlike threads on rear end
Putative identification Arthropoda Insecta Orthoptera Gryllidae Gryllus Gryllus pennsylvanicus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 11 on gel image.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Band seen in arthropod CO1 gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Gryllus pennsylvanicus was found to be negative for Wolbachia.
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