JWO 14 – Duskwing

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

Flying around in HACC Pollinator Garden

Dark brown, tiny whitish spots on wings.
Putative identification Arthropoda Insecta Lepidoptera Hesperiidae Erynnis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 14 on gel image.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Band found in Arthropod CO1 gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Erynnis was found to be negative for Wolbachia.
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