JWO 16 – Planthopper

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

On grass in HACC Pollinator Garden.

Leaf green, giant wings compared to body, piercing mouth parts.
Putative identification Arthropoda Insecta Hemiptera Acanaloniidae Acanalonia Acanalonia conica

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 16 on gel image.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Vivid band on CO1.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Acanalonia conica was found to be negative for Wolbachia.
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