JWO 18 – Boneset Borer Moth

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

On flower in HACC Pollinator Garden.

Yellow head, big black eyes, single yellow stripe w/ black dot
Putative identification Arthropoda Insecta Lepidoptera Sesiidae Carmenta Carmenta pyralidiformis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 18 on gel image.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Vivid CO1 band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Carmenta pyralidiformis was found to be negative for Wolbachia.
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