November 2, 2026

Sample information
Picture
Entry by: Kenyanna G.
Location
Approximate location: 31.97, -81.16
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • The Method Used: Collection Tray and Gloves

 

  • Location of Collection: Between The Science Center and Lane Library close to where the bench is at

 

  • Description of the Environment: Leaves
  • Soil
  • Sticks
  • Trees
  • Vegetation
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I carefully followed all instructions and took my time to complete the labs accurately.
Wolbachia 16S sequence
Arthropod COI sequence
TTGNAACAGCTCTTANAGTTTTAATTCNAATCAAATTAGGC CAACCAGGATCTTTAATTGGAGATGATCAAATTTATAATGTTATTGTTACNGCTCACGCATTCGTTATAATTTTCTTCAT AGTTATGCCTATTATAATTGGTGGNTTTGGAAACTGGTTAGTTCCTTTAATATTANGAGCCCCGGATATANCCTTCCCCC NTCTTAATAATATAANTTTTTGATTACTTCCTCCCCCCNTCACATTACTTTTATCTANAAATATAGTANAAAGAGGAGCA GGAACNGGATGAACTGTAT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be negative for Wolbachia.
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