Ant

September 7, 2023

Sample information
Picture
Location
Collection date 10/15/2020
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

– very small

– black body

– missing two legs
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

– used same controls throughout the gel tests

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

– bands were not very clear/bright
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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