Sample information

Photos by: Scottie H.
Collection date 09/01/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy

Was very docile and was a little startled after collection.

Putative identification Arthropoda Hexapoda Insecta Coleoptera


Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I believe our controls worked and my sample was positive for Wolbachia, I do not believe any mistakes were made to my sample. But the arthropod control didn’t have a band for arthropod and we do not know why.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be postive for Wolbachia.
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