DEMALJ blattodea

November 3, 2026

Sample information
Picture
Entry by: Alara J
Location
Approximate location: 31.97, -81.16
Collection date 08/25/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found underneath a shaded tree in leaf litter.
Putative identification Arthropoda Insecta Blattodea Blaberidae

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Lack of band at the proper level corresponding to the presence of Wolbachia. Controls exhibited correct results, and the insect specimen, present in track 5, exhibits only Insect CO1 gene.
Wolbachia 16S sequence
Arthropod COI sequence
GAATATTAATCCGAGCAGAACTAAATCAACCTGGTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGGCTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTATCTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGAACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGTAGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAGGTGCCGTAAATTTTATTTCNACAATTATTAACATGAAACCNATTAATATAAGTCCTGAACGAATTCCTTTATTTGNTTGGGTCAGTANGTANNACTGCANNATNANNATTATTATCCNNACCAGTNCNTGCAGGTGCAATTACNATATTATTAACAGACCGAAATTTNNTANTTCATTTTTNANNTGCNNNGGANGTGATCCTATTTNATATCAACATTTATTTTNATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blaberidae was found to be negative for Wolbachia.
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