November 3, 2026

Sample information
Picture
Entry by: Hazel M
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Description of the environment: Pathway with trees and diverse shrubbery. ​

Method of collection: Pick up and sort​

Results (Assumed):Lovebug​
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

When looking at the gel electrophoresis it fails to match up with the positive insect we have.
Wolbachia 16S sequence
Arthropod COI sequence
AGTATATTAATTCGAACAGAATTAAGC CATCCAGGATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTTTTTTTAT AGTTATACCAATTATAATTGGTGGATTTGGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTC GAATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGGGGCT GGTACTGGATGAACAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTTTTC TCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAA TTACATTTGATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCCAGTTTTA GCCGGGGCTATTACTATACTATTAACAGATCGAAATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCTAT TTTATACCAACATTTATTTTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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