CON-ECT Orthoptera

November 4, 2026

Sample information
Picture
Location
Approximate location: 31.97, -81.16
Collection date 08/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am highly confident as my gel electrophoresis results came out clear that there was a Wolbachia strain present.
Wolbachia 16S sequence
n/a
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TCGAACAGAATTANGNCN ACCNGGATCATTNNTTGNAGATGATCAAATTTATNNNGNANTTGTTACTGCTCACGCTTTTGTAATAATTTTTTTTATAG TAATACCAATTATNATTGGTGGATTTGGAAATTGATTANTTCCTTTAATATTANNAGCTCCNGATATANCTTTCCCTCGA ATAAATAATATAAGATTTTGATTATTACCNCCTTCTTTANCTCTCCTATTATCCANAAGATTANTANAAAACGGNGCAGG AACCGGATGAACAGTGTACCCTCCACTATCATCNCTNATTGNCCACAAAGGTGCATCCGTTGATCTTGCNATTTTTTCTC TTCATTTANNTGGAATTTCATCAATTTTANGAGCAATTAATTTTATTTCTACTATAATTAATATACGANCTCCNNGTATA NNTTTAGATCAAACTCCTNTATTNGNATGAGCTGTANGTATTACNNCCCTTCTTCTTCTTTTATCTCTTCCNNNTCTANC ANGAGCTATNACNATACTTTTANCANANCGNAATTTANATACTTCTTTTTNTGNNNNTGNTGGANGNNGAGANCCTATTT TATATC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be postive for Wolbachia.
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